Next morning. Compare the color of the starter liquid (not the foam) in this 50ml flask to yesterday. The microscopic cream-colored yeast are multiplying and their increasing numbers makes the starter look lighter.
There are technical ways of judging when to step up the starter volume but I usually go by that color change.
So I decanted the 20ml of starter from that 50ml flask into about 110ml of fresh, sterile starter in a 230ml flask. Total volume is now 130ml or so and it looks darker again because the current numbers of yeast can't cloud that volume of starter. By tomorrow we should be able to step up again. I can feel your anticipation.
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